Alpha-2-Macroglobulin Monoclonal antibody, PBS Only

Alpha-2-Macroglobulin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B11F7

Cat no : 66126-1-PBS

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

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Validation Data Gallery

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  WB analysis of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66126-1-Ig (same clone as 66126-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66126-1-Ig (same clone as 66126-1-PBS)

  IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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Product Information

66126-1-PBS targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag19245
• Full Name: alpha-2-macroglobulin
• Calculated Molecular Weight: 1474 aa, 163 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC040071
• Gene Symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.