Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4B11F7

Cat no : 66126-1-Ig

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

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Validation Data Gallery

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  WB analysis of HepG2 using 66126-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66126-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 66126-1-Ig

  IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug.

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  IHC staining of human liver using 66126-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66126-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66126-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:32000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66126-1-Ig targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag19245
• Full Name: alpha-2-macroglobulin
• Calculated Molecular Weight: 1474 aa, 163 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC040071
• Gene Symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• RRID: AB_2881525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Publications

Species	Application	Title
human	IHC	Am J Cancer Res; Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers; Authors - Mateusz Olbromski
mouse	WB	Front Physiol; Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study; Authors - Balamurugan Packialakshmi