Alpha-1-Antitrypsin Recombinant antibody

Alpha-1-Antitrypsin Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230109A8

Cat no : 82918-1-RR

Synonyms

A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT

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Validation Data Gallery

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  WB analysis using 82918-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82918-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82918-1-RR against Human Alpha-1-Antitrypsin were performed. The affinity constant is 0.719 nM.

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Tested Applications

• Positive WB detected in: human liver tissue, human heart tissue, human saliva, human placenta tissue, human urine

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82918-1-RR targets Alpha-1-Antitrypsin in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Alpha-1-Antitrypsin fusion protein Ag9369
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015642
• Gene Symbol: Alpha 1-Antitrypsin
• Gene ID (NCBI): 5265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.