Alpha-1-Antitrypsin Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Alpha-1-Antitrypsin Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230109A8

Cat no : 82918-1-PBS

Synonyms

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

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Validation Data Gallery

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  WB analysis using 82918-1-RR (same clone as 82918-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

  WB result of Alpha-1-Antitrypsin antibody (82918-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha-1-Antitrypsin transfected HepG2 cells. This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 82918-1-RR (same clone as 82918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82918-1-RR (same clone as 82918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-1-Antitrypsin Recombinant antibody (82918-1-RR, Clone:230109A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82918-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82918-1-RR against Human Alpha-1-Antitrypsin were performed. The affinity constant is 0.719 nM.

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Product Information

82918-1-PBS targets Alpha-1-Antitrypsin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Alpha-1-Antitrypsin fusion protein Ag9369
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015642
• Gene Symbol: Alpha 1-Antitrypsin
• Gene ID (NCBI): 5265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.