CoraLite®594-conjugated Aldolase C Monoclonal antibody

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  IF Staining of HepG2 using CL594-66120

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Aldolase C antibody (CL594-66120, Clone: 5H7A5 ) at dilution of 1:200.

Aldolase C Monoclonal Antibody for IF/ICC

Cat No. CL594-66120

Clone No.5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66120 targets Aldolase C in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Aldolase C fusion protein Ag6779
• Full Name: aldolase C, fructose-bisphosphate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC003613
• Gene Symbol: Aldolase C
• Gene ID (NCBI): 230
• RRID: AB_2919950
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P09972
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).