Albumin Recombinant antibody, PBS Only

Albumin Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K1

Cat no : 82783-6-PBS

Synonyms

ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

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Validation Data Gallery

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  WB analysis using 82783-6-RR (same clone as 82783-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82783-6-PBS targets Albumin in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Albumin fusion protein Ag9569
• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: ALB
• Gene ID (NCBI): 213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)