Filter: AllWBIHCIF/ICC

WB analysis of human adipose using 66239-1-Ig (same clone as 66239-1-PBS)
human adipose tissue were subjected to SDS PAGE followed by western blot with 66239-1-Ig (ADIPOQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of rat brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse skin using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse skin using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of rat brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IHC staining of mouse brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.
IF Staining of 3T3-L1 using 66239-1-Ig (same clone as 66239-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using Adiponectin antibody (66239-1-Ig, Clone: 5D8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

Adiponectin Monoclonal antibody, PBS Only

Adiponectin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66239-1-PBS

Clone No.5D8A7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 5D8A7

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66239-1-PBS targets Adiponectin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Adiponectin fusion protein Ag17383 Predict reactive species
Full Name	adiponectin, C1Q and collagen domain containing
Calculated Molecular Weight	244 aa, 26 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC096308
Gene Symbol	Adiponectin
Gene ID (NCBI)	9370
RRID	AB_2881628
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q15848
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.