Adenosine A1 Receptor Polyclonal antibody, PBS Only

Adenosine A1 Receptor Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 20332-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse eye using 20332-1-AP (same clone as 20332-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 20332-1-AP (same clone as 20332-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 20332-1-AP (same clone as 20332-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 20332-1-AP (same clone as 20332-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

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Product Information

20332-1-PBS targets Adenosine A1 Receptor in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Adenosine A1 Receptor fusion protein Ag14092
• Full Name: adenosine A1 receptor
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC026340
• Gene Symbol: Adenosine A1 Receptor
• Gene ID (NCBI): 134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.