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Adenosine A1 Receptor Polyclonal antibody

Adenosine A1 Receptor Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20332-1-AP

Synonyms

RDC7, ADORA1, Adenosine receptor A1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    WB analysis of mouse eye using 20332-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse brain using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse brain using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse cerebellum using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse cerebellum using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    FC experiment of SH-SY5Y using 20332-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20332-1-AP targets Adenosine A1 Receptor in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Adenosine A1 Receptor fusion protein Ag14092
Full Name adenosine A1 receptor
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC026340
Gene Symbol Adenosine A1 Receptor
Gene ID (NCBI) 134
RRIDAB_2878673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

Protocols

Product Specific Protocols
WB protocol for Adenosine A1 Receptor antibody 20332-1-APDownload protocol
IHC protocol for Adenosine A1 Receptor antibody 20332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Biol Toxicol

Adenosine kinase protects against acetaminophen-induced acute liver injury by activating autophagy in hepatocytes

Authors - Chuanxin Zhang
View Article
human

PLoS One

Discovery of a potent, selective, and tumor-suppressing antibody antagonist of adenosine A2A receptor

Authors - Linya Wang
View Article
mouseWB,IF

J Orthop Translat

CD73 alleviates osteoarthritis by maintaining anabolism and suppressing catabolism of chondrocytes extracellular matrix

Authors - Hu Guo
View Article