CoraLite® Plus 488-conjugated Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D18

Cat no : CL488-83095

Synonyms

HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4

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Validation Data Gallery

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  IF Staining of Caco-2 using CL488-83095

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 Acetyl-Histone H4 (Lys12) antibody (CL488-83095, Clone: 1D18 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83095 targets Acetyl-Histone H4 (Lys12) in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.