Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.
Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 82902-1-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 82902-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Dot Blot experiment of peptide using 82902-1-RR
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys27) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row.
Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS).
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mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive Dot Blot detected in
peptide
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:19600
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
DOT BLOT
DOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.
Protocols
Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys27) antibody 82902-1-RR
Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 82902-1-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 82902-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
DOT BLOT Figures
Dot Blot experiment of peptide using 82902-1-RR
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys27) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row.
Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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