ATX3,ATXN3 Monoclonal antibody, PBS Only

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat no : 67057-1-PBS

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

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Validation Data Gallery

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  WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67057-1-Ig (same clone as 67057-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67057-1-Ig (same clone as 67057-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67057-1-Ig (same clone as 67057-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67057-1-Ig (same clone as 67057-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67057-1-Ig (same clone as 67057-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67057-1-PBS targets ATX3,ATXN3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATX3,ATXN3 fusion protein Ag6627
• Full Name: ataxin 3
• Calculated Molecular Weight: 370 aa, 43 kDa
• Observed Molecular Weight: 41-45 kDa
• GenBank Accession Number: BC033711
• Gene Symbol: ATXN3
• Gene ID (NCBI): 4287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.