ATRX Polyclonal antibody, PBS Only

ATRX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20495-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

  A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

---

Product Information

20495-1-PBS targets ATRX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATRX fusion protein Ag13984
• Full Name: alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
• Calculated Molecular Weight: 2492 aa, 283 kDa
• Observed Molecular Weight: 280-300 kDa
• GenBank Accession Number: BC002521
• Gene Symbol: ATRX
• Gene ID (NCBI): 546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).