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ATRX Polyclonal antibody

ATRX Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20495-1-AP

Synonyms

RAD54L, RAD54, MRXHF1, EC:3.6.4.12, ATR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using human ATRX Polyclonal antibody (20495-1-AP)

    WB analysis using 20495-1-AP

    A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using human ATRX Polyclonal antibody (20495-1-AP)

    IHC staining of rat brain using 20495-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using human ATRX Polyclonal antibody (20495-1-AP)

    IHC staining of rat brain using 20495-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATRX Polyclonal antibody (20495-1-AP)

    IF Staining of A549 using 20495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATRX Polyclonal antibody (20495-1-AP)

    IF Staining of A549 using 20495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20495-1-AP targets ATRX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATRX fusion protein Ag13984
Full Name alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
Calculated Molecular Weight 2492 aa, 283 kDa
Observed Molecular Weight280-300 kDa
GenBank Accession NumberBC002521
Gene Symbol ATRX
Gene ID (NCBI) 546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).

Protocols

Product Specific Protocols
WB protocol for ATRX antibody 20495-1-APDownload protocol
IHC protocol for ATRX antibody 20495-1-APDownload protocol
IF protocol for ATRX antibody 20495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Roy (Verified Customer) (06-13-2024)

Works great to detect ATRX in Jurkat/HeLa cells by WB (1/1000 dilution- Overnight incubation at 4°C).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa/Jurkat cells