ATPAF2 Monoclonal antibody, PBS Only (Capture)

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9A9

Cat no : 68351-1-PBS

Synonyms

ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9

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Validation Data Gallery

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  WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50625-1

  Cytometric bead array standard curve of MP50625-1, ATPAF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68351-1-PBS. Detection antibody: 68351-2-PBS. Standard:Ag29065. Range: 0.098-6.25 ng/mL.

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Product Information

68351-1-PBS targets ATPAF2 as part of a matched antibody pair:

MP50625-1: 68351-1-PBS capture and 68351-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATPAF2 fusion protein Ag29065
• Full Name: ATP synthase mitochondrial F1 complex assembly factor 2
• Calculated Molecular Weight: 289 aa, 33 kDa
• Observed Molecular Weight: ~30 kDa
• GenBank Accession Number: BC032126
• Gene Symbol: ATPAF2
• Gene ID (NCBI): 91647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)