CoraLite® Plus 488-conjugated ATP6V1B1 Monoclonal antibody

ATP6V1B1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10A8

Cat no : CL488-68219

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-68219

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ATP6V1B1 antibody (CL488-68219, Clone: 1G10A8 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68219 targets ATP6V1B1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V1B1 fusion protein Ag7405
• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC063411
• Gene Symbol: ATP6V1B1
• Gene ID (NCBI): 525
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.