ATP5J2 Monoclonal antibody, PBS Only

ATP5J2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C3

Cat no : 68128-1-PBS

Synonyms

ATP5J2, ATP5JL

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Validation Data Gallery

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  WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 68128-1-Ig (same clone as 68128-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 68128-1-Ig (same clone as 68128-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68128-1-PBS targets ATP5J2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5J2 fusion protein Ag8811
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
• Calculated Molecular Weight: 5-11 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC003678
• Gene Symbol: ATP5J2
• Gene ID (NCBI): 9551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.