ATP5J2 Monoclonal antibody

ATP5J2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B3C3

Cat no : 68128-1-Ig

Synonyms

ATP5J2, ATP5JL

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Validation Data Gallery

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  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 68128-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 68128-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat heart tissue, rabbit heart tissue, LNCaP cells, mouse heart tissue, mouse liver tissue, rat liver tissue, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68128-1-Ig targets ATP5J2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5J2 fusion protein Ag8811
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
• Calculated Molecular Weight: 5-11 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC003678
• Gene Symbol: ATP5J2
• Gene ID (NCBI): 9551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration; Authors - Wenya Ma