ATP5E Polyclonal antibody, PBS Only

• 

  WB analysis using 15408-1-AP (same clone as 15408-1-PBS)

  Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15408-1-AP (same clone as 15408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15408-1-AP (same clone as 15408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

ATP5E Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 15408-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

15408-1-PBS targets ATP5E in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5E fusion protein Ag7672
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
• Calculated Molecular Weight: 6 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC001690
• Gene Symbol: ATP5E
• Gene ID (NCBI): 514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56381
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).