ATP5E Polyclonal antibody

ATP5E Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15408-1-AP

Synonyms

ATPE, ATP5F1E, ATP synthase F1 subunit epsilon

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Validation Data Gallery

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  WB analysis using 15408-1-AP

  Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15408-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15408-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, 37℃ incubated mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15408-1-AP targets ATP5E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5E fusion protein Ag7672
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
• Calculated Molecular Weight: 6 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC001690
• Gene Symbol: ATP5E
• Gene ID (NCBI): 514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).