ATP5C1 Monoclonal antibody, PBS Only

ATP5C1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5A2

Cat no : 60284-1-PBS

Synonyms

F-ATPase gamma subunit, ATP5F1C, ATP5CL1, ATP5C, ATP synthase F1 subunit gamma

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Validation Data Gallery

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  WB analysis of human heart using 60284-1-Ig (same clone as 60284-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60284-1-Ig (same clone as 60284-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60284-1-Ig (same clone as 60284-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60284-1-Ig (ATP5C1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 60284-1-Ig (same clone as 60284-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5C1 (60284-1-Ig, Clone:1B5A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

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Product Information

60284-1-PBS targets ATP5C1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5C1 fusion protein Ag1343
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000931
• Gene Symbol: ATP5C1
• Gene ID (NCBI): 509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).