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ATP5A1 Monoclonal antibody

ATP5A1 Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat, monkey and More (1)







Cat no : 66037-1-Ig


ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:150-1:600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66037-1-Ig targets ATP5A1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATP5A1 fusion protein Ag8119
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC064562
Gene Symbol ATP5A1
Gene ID (NCBI) 498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.


Product Specific Protocols
WB protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IHC protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IF protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IP protocol for ATP5A1 antibody 66037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi

Nat Commun

The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters

Authors - Xiaolong Lv

Dev Cell

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.

Authors - Deguan Lv


AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Authors - Zhenhong Ni


CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis

Authors - Meng Ding


Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Authors - Maxence Le Vasseur


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Marina (Verified Customer) (09-12-2022)

Mitochondrial fractions were obtained from whole cell lysates of 2 immortalized human breast cancer cell lines using a commercial kit, leading to pure mitochondrial fractions (ATP5A1 positive) and fractions comprising the cytosols minus the mitochondria (ATP5A1 negative). Primary antibody incubation was performed overnight at 4ºC. Ponceau S is used as total protein loading control.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human breast cancer cells
ATP5A1 Antibody Western Blot validation (1:5000 dilution) in Human breast cancer cells (Cat no:66037-1-Ig)

Cassandr (Verified Customer) (02-10-2022)

Consistently works antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HULEC
ATP5A1 Antibody Immunofluorescence validation (1:250 dilution) in HULEC (Cat no:66037-1-Ig)