ATP5A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATP5A1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10H3

Cat no : 66037-1-PBS

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

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Validation Data Gallery

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  WB analysis using 66037-1-Ig (same clone as 66037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

  WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

  Western blot analysis of ATP5A1 in various tissues and cell lines using Proteintech antibody 66037-1-Ig at the dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66037-1-Ig (same clone as 66037-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 66037-1-Ig (same clone as 66037-1-PBS)

  IP result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

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Product Information

66037-1-PBS targets ATP5A1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5A1 fusion protein Ag8119
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC064562
• Gene Symbol: ATP5A1
• Gene ID (NCBI): 498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.