ATP2A1 Recombinant antibody

ATP2A1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241500D12

Cat no : 84213-5-RR

Synonyms

SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1, ATP2A

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Validation Data Gallery

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  WB analysis using 84213-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84213-5-RR (ATP2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 84213-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84213-5-RR (ATP2A1 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84213-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84213-5-RR against Human ATP2A1 were performed. The affinity constant is 6.90 nM.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84213-5-RR is ATP2A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP2A1 fusion protein Ag17944
• Full Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calculated Molecular Weight: 1001 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC037354
• Gene Symbol: ATP2A1
• Gene ID (NCBI): 487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).