ATP2A1 Recombinant antibody, PBS Only

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  WB analysis using 84213-5-RR (same clone as 84213-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84213-5-RR (ATP2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84213-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84213-5-RR (same clone as 84213-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84213-5-RR (ATP2A1 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84213-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84213-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84213-5-RR against Human ATP2A1 were performed. The affinity constant is 6.90 nM.

ATP2A1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84213-5-PBS

Clone No.241500D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1, ATP2A

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Product Information

84213-5-PBS targets ATP2A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP2A1 fusion protein Ag17944
• Full Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calculated Molecular Weight: 1001 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC037354
• Gene Symbol: ATP2A1
• Gene ID (NCBI): 487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14983
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).