ATP1B3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATP1B3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9C1

Cat no : 67554-1-PBS

Synonyms

ATP1B3, ATPB 3, CD298, FLJ29027

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Validation Data Gallery

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  WB analysis of Jurkat using 67554-1-Ig (same clone as 67554-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 67554-1-Ig (same clone as 67554-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67554-1-Ig (same clone as 67554-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67554-1-Ig (same clone as 67554-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67554-1-Ig (same clone as 67554-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67554-1-PBS targets ATP1B3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP1B3 fusion protein Ag30127
• Full Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: BC011835
• Gene Symbol: ATP1B3
• Gene ID (NCBI): 483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).