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  • Featured Product
  • KD/KO Validated

ATP1B3 Monoclonal antibody

ATP1B3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9C1

Cat no : 67554-1-Ig

Synonyms

Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, CD298, ATPB-3, ATPB 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of Jurkat using 67554-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of THP-1 using 67554-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of human placenta using 67554-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of HeLa using 67554-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of HepG2 using 67554-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human colon using 67554-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human colon using 67554-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human lung cancer using 67554-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human lung cancer using 67554-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IF Staining of A431 using 67554-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1B3 antibody (67554-1-Ig, Clone: 1H9C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67554-1-Ig targets ATP1B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1B3 fusion protein Ag30127
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
RRIDAB_2882768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Protocols

Product Specific Protocols
WB protocol for ATP1B3 antibody 67554-1-IgDownload protocol
IHC protocol for ATP1B3 antibody 67554-1-IgDownload protocol
IF protocol for ATP1B3 antibody 67554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.

Authors - Shanshan Lu
  • KD Validated
View Article