ATP1A2-Specific Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 55179-1-AP (same clone as 55179-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55179-1-AP (same clone as 55179-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 55179-1-AP (same clone as 55179-1-PBS)

  IP result of anti-ATP1A2-Specific (IP:55179-1-AP, 4ug; Detection:55179-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ATP1A2-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55179-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ATP1A2, Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2

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Product Information

55179-1-PBS targets ATP1A2-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_000702
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• RRID: AB_2881284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50993
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.