ATP1A1/2 Monoclonal antibody

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8G1

Cat no : 68505-1-Ig

Synonyms

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

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Validation Data Gallery

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  WB analysis using 68505-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of SK-BR-3 using 68505-1-Ig

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 68505-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68505-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of C2C12 using 68505-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: rat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68505-1-Ig targets ATP1A1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP1A1/2 fusion protein Ag10489
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 1020 aa, 112 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC052271
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.