ATP1A1/2 Monoclonal antibody, PBS Only

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  WB analysis using 68505-1-Ig (same clone as 68505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of SK-BR-3 using 68505-1-Ig (same clone as 68505-1-PBS)

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 68505-1-Ig (same clone as 68505-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 68505-1-Ig (same clone as 68505-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 68505-1-Ig (same clone as 68505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68505-1-PBS

Clone No.3D8G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

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Product Information

68505-1-PBS targets ATP1A1/2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP1A1/2 fusion protein Ag10489
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 1020 aa, 112 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC052271
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50993
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.