CoraLite® Plus 488-conjugated ATP1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP1A1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14418

Synonyms

Na+/K+ ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1

---

Validation Data Gallery

• 

  IF Staining of Caco-2 using CL488-14418

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 ATP1A1 antibody (CL488-14418) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14418 targets ATP1A1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1A1 fusion protein Ag5763
• Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC050359
• Gene Symbol: ATP1A1
• Gene ID (NCBI): 476
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.