ATOH1 Polyclonal antibody

ATOH1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, chIP, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (7)

Cat no : 21215-1-AP

Synonyms

MATH 1, Helix-loop-helix protein hATH-1, HATH1, Class A basic helix-loop-helix protein 14, bHLHa14

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Validation Data Gallery

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  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21215-1-AP (ATOH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, HepG2 cells, mouse small intestine tissue, mouse brain tissue, rat brain tissue
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 16 publications below
• ChIP: See 1 publications below

Product Information

21215-1-AP targets ATOH1 in WB, IHC, IF/ICC, IF-P, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATOH1 fusion protein Ag15615
• Full Name: atonal homolog 1 (Drosophila)
• Calculated Molecular Weight: 354 aa, 38 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC069594
• Gene Symbol: ATOH1
• Gene ID (NCBI): 474
• RRID: AB_10733126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

Publications

Species	Application	Title
human	IF	Cell Res; Decoding the spatiotemporal regulation of transcription factors during human spinal cord development; Authors - Yingchao Shi
human	IF	Immunity; IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.; Authors - Xun Lin
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
mouse	IHC	J Clin Invest; Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.; Authors - Patrick J Atkinson
mouse	IF	Proc Natl Acad Sci U S A; Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation; Authors - Tianli Qin
human	WB	Oncogene; Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment.; Authors - Yoshihiro Morimoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicole (Verified Customer) (08-08-2024)

Works really well both in Western Blot and Immunofluorescence

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500

FH

Yin (Verified Customer) (12-20-2021)

It did not work.

• Applications: Immunofluorescence
• Cell Tissue Type: Cerebella granule cell

FH

Stephen (Verified Customer) (11-04-2020)

ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Embryonic chicken inner ear tissue

ATOH1 Antibody Immunofluorescence validation (1:300 dilution) in Embryonic chicken inner ear tissue (Cat no:21215-1-AP)

FH

Roland (Verified Customer) (09-17-2020)

We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.

• Applications: Western Blot
• Cell Tissue Type: human cancer cells

FH

Biren (Verified Customer) (08-31-2020)

I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human glioblastoma stem cells

ATOH1 Antibody Immunofluorescence validation (1/500 dilution) in human glioblastoma stem cells (Cat no:21215-1-AP)

FH

Erithelgi (Verified Customer) (09-25-2019)

The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: medulloblastoma

FH

Nicole (Verified Customer) (07-23-2019)

Used to stain cultured stem cells for differentiation markers.

• Applications: Immunohistochemistry, Immunofluorescence,
• Cell Tissue Type: Stem Cell culture