ATM Recombinant antibody, PBS Only

ATM Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240560F5

Cat no : 83608-5-PBS

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560F5

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Validation Data Gallery

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  WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83608-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

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Product Information

The immunogen of 83608-5-PBS is ATM Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATM fusion protein Ag25920
• Full Name: ataxia telangiectasia mutated
• GenBank Accession Number: BC137169
• Gene Symbol: ATM
• Gene ID (NCBI): 472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.