ATIC Recombinant antibody, PBS Only

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  WB analysis using 84798-5-RR (same clone as 84798-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84798-5-RR (ATIC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATIC Recombinant antibody (84798-5-RR, Clone:242324C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

ATIC {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 84798-5-PBS

Clone No.242324C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase

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Product Information

84798-5-PBS targets ATIC in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATIC fusion protein Ag1052
• Full Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC008879
• Gene Symbol: ATIC
• Gene ID (NCBI): 471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31939
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC, also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. It has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression (PMID: 35251351).