ATG4D Recombinant antibody, PBS Only (Detector)

ATG4D Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241035G8

Cat no : 83951-4-PBS

Synonyms

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

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Validation Data Gallery

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  WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00902-3

  Cytometric bead array standard curve of MP00902-3, ATG4D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83951-1-PBS. Detection antibody: 83951-4-PBS. Standard: Ag10177. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

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Product Information

83951-4-PBS targets ATG4D as part of a matched antibody pair:

MP00902-3: 83951-1-PBS capture and 83951-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATG4D fusion protein Ag10177
• Full Name: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC068992
• Gene Symbol: ATG4D
• Gene ID (NCBI): 84971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.