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ATG4D Recombinant antibody

ATG4D Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241035G8

Cat no : 83951-4-RR

Synonyms

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ATG4D Recombinant antibody (83951-4-RR)

    WB analysis of A549 using 83951-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ATG4D Recombinant antibody (83951-4-RR)

    IHC staining of rat liver using 83951-4-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83951-4-RR

    Affinity and Kinetic Characterization of 83951-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83951-4-RR is ATG4D Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG4D fusion protein Ag10177
Full Name ATG4 autophagy related 4 homolog D (S. cerevisiae)
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC068992
Gene Symbol ATG4D
Gene ID (NCBI) 84971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

Protocols

Product Specific Protocols
WB protocol for ATG4D antibody 83951-4-RRDownload protocol
IHC protocol for ATG4D antibody 83951-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols