ATG16L1 Monoclonal antibody

ATG16L1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G7

Cat no : 67943-1-Ig

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30



Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67943-1-Ig targets ATG16L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG16L1 fusion protein Ag14881
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight68-75 kDa
GenBank accession numberBC000061
Gene symbol ATG16L1
Gene ID (NCBI) 55054
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Protocols

Product Specific Protocols
WB protocol for ATG16L1 antibody 67943-1-IgDownload protocol
IF protocol for ATG16L1 antibody 67943-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols