ATG12 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11264-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 11264-1-AP (same clone as 11264-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11264-1-AP (same clone as 11264-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)

  Immunofluorescent analysis of PC-3 cells using 11264-1-AP (ATG12 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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Product Information

11264-1-PBS targets ATG12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG12 fusion protein Ag1791
• Full Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC012266
• Gene Symbol: ATG12
• Gene ID (NCBI): 9140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.