CoraLite® Plus 488-conjugated ATF6 Monoclonal antibody

• Featured Product
• KD/KO Validated

ATF6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B7E4

Cat no : CL488-66563

Synonyms

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66563

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ATF6 antibody (CL488-66563, Clone: 3B7E4 ) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66563

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ATF6 antibody (CL488-66563, Clone: 3B7E4 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66563 targets ATF6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATF6 fusion protein Ag21456
• Full Name: activating transcription factor 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC014969
• Gene Symbol: ATF6
• Gene ID (NCBI): 22926
• RRID: AB_2919344
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.