ATF6 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATF6 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B7E4

Cat no : 66563-1-PBS

Synonyms

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

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Validation Data Gallery

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  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66563-1-Ig (same clone as 66563-1-PBS)

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66563-1-Ig (same clone as 66563-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66563-1-Ig (same clone as 66563-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66563-1-Ig (same clone as 66563-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66563-1-Ig (same clone as 66563-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF6 antibody (66563-1-Ig, Clone: 3B7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (66563-1-Ig, Clone:3B7E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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Product Information

66563-1-PBS targets ATF6 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATF6 fusion protein Ag21456
• Full Name: activating transcription factor 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC014969
• Gene Symbol: ATF6
• Gene ID (NCBI): 22926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.