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ATF5 Monoclonal antibody, PBS Only

ATF5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5B6

Cat no : 67066-1-PBS

Synonyms

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATF5 Monoclonal antibody (67066-1-Ig)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATF5 Monoclonal antibody (67066-1-Ig)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67066-1-PBS targets ATF5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATF5 fusion protein Ag7214
Full Name activating transcription factor 5
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC005174
Gene Symbol ATF5
Gene ID (NCBI) 22809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).