ATF5 Monoclonal antibody

ATF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1E5B6

Cat no : 67066-1-Ig

Synonyms

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx

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Validation Data Gallery

• 

  WB analysis using 67066-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67066-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67066-1-Ig targets ATF5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATF5 fusion protein Ag7214
• Full Name: activating transcription factor 5
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC005174
• Gene Symbol: ATF5
• Gene ID (NCBI): 22809
• RRID: AB_2882376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).

Publications

Species	Application	Title
human,mouse	WB	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	WB	Toxicol Lett; Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction; Authors - Ying Yang
human	WB	FEBS Open Bio; Knockdown of TMEM160 leads to an increase in reactive oxygen species generation and the induction of the mitochondrial unfolded protein response; Authors - Kosei Yamashita