CoraLite® Plus 488-conjugated ATF4 Recombinant antibody

ATF4 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230306B5

Cat no : CL488-81798-2

Synonyms

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Validation Data Gallery

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  IF Staining of HeLa using CL488-81798-2

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CoraLite® Plus 488 ATF4 antibody (CL488-81798-2, Clone: 230306B5 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: Tunicamycin treated HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81798-2 targets ATF4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATF4 fusion protein Ag1279
• Full Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC022088
• Gene Symbol: ATF4
• Gene ID (NCBI): 468
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. This antibody is a mouse monoclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 45-50 kDa ATF4 protein and its phosphorylated forms (50 kDa).