ATF4 Recombinant antibody, PBS Only

ATF4 Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N3

Cat no : 81798-1-PBS

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB

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Validation Data Gallery

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  WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

  Non-treated HEK-293 and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

  Non-treated HeLa and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

  IP result of anti-ATF4 (IP:81798-1-RR, 4ug; Detection:81798-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81798-1-PBS targets ATF4 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATF4 fusion protein Ag1279
• Full Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022088
• Gene Symbol: ATF4
• Gene ID (NCBI): 468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.