ATF4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATF4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4C5

Cat no : 60035-1-PBS

Synonyms

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 60035-1-Ig (same clone as 60035-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60035-1-Ig (same clone as 60035-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60035-1-Ig (same clone as 60035-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60035-1-Ig (same clone as 60035-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 60035-1-Ig(ATF4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 60035-1-Ig(ATF4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60035-1-Ig(ATF4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60035-1-Ig (same clone as 60035-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

---

Product Information

60035-1-PBS targets ATF4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATF4 fusion protein Ag1279
• Full Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC022088
• Gene Symbol: ATF4
• Gene ID (NCBI): 468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)