ATF2 Polyclonal antibody, PBS Only

ATF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11908-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

  IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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Product Information

11908-1-PBS targets ATF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF2 fusion protein Ag2581
• Full Name: activating transcription factor 2
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 60 kDa, 64 kDa
• GenBank Accession Number: BC026175
• Gene Symbol: ATF2
• Gene ID (NCBI): 1386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).