CoraLite® Plus 488-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7C11

Cat no : CL488-66346

Synonyms

ARSA, ARSA I, ARSA1, Arsenical pump driving ATPase, Arsenite stimulated ATPase, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66346

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66346 (ASNA1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66346 targets ASNA1 in IF/ICC applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASNA1 fusion protein Ag7936
• Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Calculated Molecular Weight: 38.7 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC002651
• Gene Symbol: ASNA1
• Gene ID (NCBI): 439
• RRID: AB_2883314
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.