ASIC1 Recombinant antibody

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  WB analysis using 85089-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85089-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ASIC1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85089-1-RR

Clone No.242749B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1, 242749B11

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse brain tissue, LNCaP cells, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85089-1-RR targets ASIC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ASIC1 fusion protein Ag25941
• Full Name: amiloride-sensitive cation channel 2, neuronal
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: NM_001095
• Gene Symbol: ASIC1
• Gene ID (NCBI): 41
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78348
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).