ASIC1 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85089-1-RR (same clone as 85089-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85089-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85089-1-RR (same clone as 85089-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85089-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01789-2

  Cytometric bead array standard curve of MP01789-2, ASIC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85089-1-PBS. Detection antibody: 85089-2-PBS. Standard: Ag25941. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01789-1

  Cytometric bead array standard curve of MP01789-1, ASIC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85089-1-PBS. Detection antibody: 85089-3-PBS. Standard: Ag25941. Range: 0.313-40 ng/mL.

ASIC1 {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85089-1-PBS

Clone No.242749B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1, 242749B11

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Product Information

85089-1-PBS targets ASIC1 as part of a matched antibody pair:

MP01789-1: 85089-1-PBS capture and 85089-3-PBS detection (validated in Cytometric bead array)

MP01789-2: 85089-1-PBS capture and 85089-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ASIC1 fusion protein Ag25941
• Full Name: amiloride-sensitive cation channel 2, neuronal
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: NM_001095
• Gene Symbol: ASIC1
• Gene ID (NCBI): 41
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78348
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).