CoraLite® Plus 488-conjugated ASGR1 Recombinant antibody

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  IF Staining of rat liver using CL488-83691-3

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-83691-3, Clone: 240672C4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using CL488-83691-3

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-83691-3, Clone: 240672C4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASGR1 Recombinant Antibody for IF-P

Cat No. CL488-83691-3

Clone No.240672C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

asialoglycoprotein receptor 1, HL-1, 240672C4, ASGP R 1, ASGPR

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Tested Applications

• Positive IF-P detected in: rat liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83691-3 targets ASGR1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ASGR1 fusion protein Eg0946
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: NM_001671.4
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07306-1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.